The Animal Justice Project secretly filmed the handling of calves at the Oaklands Veterinary Center.
The film, released by the charity, showed animals being attacked and, in some cases, losing food and water for long periods of time.
The Shropshire Star made several attempts to contact the owner of the North Shropshire business, but received no response.
Some of the calves involved are understood to have come from farms that supply dairy company Muller – which supplies a supermarket chain called Sainsbury’s.
M முller had already told his suppliers to stop using the Oaklands Livestock Center, and a spokesman said: “In light of this abusive evidence, we are advising farmers to stop supplying Oaklands immediately.”
Now Sainsbury’s has expressed his concern over the reports and said he would look into it.
A spokesman for the supermarket said: “We have procedures in place to prevent these unacceptable practices and we are urgently investigating.”
The calves are understood to be collected from the animal center farms and taken away for slaughter.
Footage filmed during the investigation at the center on the Weston-Under-Red Coast near Wem showed workers kicking calves from the back of trailers while another worker appeared to be stabbing a calf.
The Animal Justice Project says the footage was captured during an undercover investigation between November and March last year.
Eddie Pauls, a lawyer for Animal Lawyer, said: “The footage collected by the Animal Justice Program calls into question the compliance of many welfare laws that farmers must follow.
“These violations need to be fully investigated and appropriate action taken. The public must be convinced that the laws enacted to protect domestic animals are not just on paper.”